Journal: International Journal of Molecular Medicine

Article Title: Isorhamnetin protects against bleomycin-induced pulmonary fibrosis by inhibiting endoplasmic reticulum stress and epithelial-mesenchymal transition

doi: 10.3892/ijmm.2018.3965

Figure Lengend Snippet: Effects of Isor on BLM-induced pulmonary endoplasmic reticulum stress markers. (A) The protein expression levels of CHOP and GRP78 were assessed by western blot analysis, and the densitometry values were normalized to β-actin. (B) The mRNA expression levels of CHOP and GRP78 were determined by reverse transcription-quantitative polymerase chain reaction. (C) Immunohistochemistry staining of CHOP in lung tissue sections (magnification, ×200 and ×400). Data are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01 and *** P<0.001, with comparisons indicated by lines. Isor, isorhamnetin; BLM, bleomycin; CHOP, DNA damage-inducible transcript 3; GRP78, 78 kDa glucose-regulating protein.

Article Snippet: Antibodies targeting vimentin (dilution, 1:1,200; YT4880), phosphorylated (p)-PERK (dilution, 1:2,000; cat. no. YP1055), DNA damage-inducible transcript 3 (DDIT3; also known as CHOP; dilution, 1:1,200; cat. no. YT0911), eukaryotic translation initiation factor 2 subunit α (eIF2α; dilution, 1:1,200; cat. no. YT1507) and p-eIF2α (dilution, 1:1,000; cat. no. YP0093) were obtained from ImmunoWay Biotechnology, Plano, TX, USA.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Immunohistochemistry, Staining, Standard Deviation

Journal: International Journal of Molecular Medicine

Article Title: Isorhamnetin protects against bleomycin-induced pulmonary fibrosis by inhibiting endoplasmic reticulum stress and epithelial-mesenchymal transition

doi: 10.3892/ijmm.2018.3965

Figure Lengend Snippet: Effects of Isor on the expression of endoplasmic reticulum stress markers in TGFβ1-induced A549 cells and HBECs. The protein expression levels of CHOP and GRP78 were assessed by western blot analysis in A549 cells and in HBECs, and the densitometry values were normalized to β-actin. Data are presented as the mean ± standard deviation (n=3). ** P<0.01 and *** P<0.001, with comparisons indicated by lines. Isor, isorhamnetin; TGFβ1, transforming growth factor β1; HBECs, human bronchial epithelial cells; CHOP, DNA damage-inducible transcript 3; GRP78, 78 kDa glucose-regulating protein.

Article Snippet: Antibodies targeting vimentin (dilution, 1:1,200; YT4880), phosphorylated (p)-PERK (dilution, 1:2,000; cat. no. YP1055), DNA damage-inducible transcript 3 (DDIT3; also known as CHOP; dilution, 1:1,200; cat. no. YT0911), eukaryotic translation initiation factor 2 subunit α (eIF2α; dilution, 1:1,200; cat. no. YT1507) and p-eIF2α (dilution, 1:1,000; cat. no. YP0093) were obtained from ImmunoWay Biotechnology, Plano, TX, USA.

Techniques: Expressing, Western Blot, Standard Deviation